Shown is a coronal section through the olfactory bulbs to visualize the vomeronasal nerve, which projects from the vomeronasal organ to the accessory olfactory bulbs. The vomeronasal nerve is stained with Erythrina Cristagalli lectin (green) while a subset of main and accessory olfactory fibers are stained with OCAM antibodies (red). A subset of vomeronasal fibers are OCAM-positive (yellow from the overlapping red and green). Cells of the olfactory bulbs are stained with Hoechst dye (blue). We have recently shown that development of vomeronasal projections requires neuropilin-2, a receptor for the axonal chemoreppellent Sema3F (See Cloutier et al., Neuron, 33:877-892, 2002).
© 2005 Department of Neuroscience, Johns Hopkins University School of Medicine