Dwight Bergles Picture Details

A confocal image of an astrocyte located in the CA1 region of the hippocampus that has been filled with the fluorescent dye Cy5-EDA through a patch pipette. Astrocytes have a complex morphology with numerous fine processes that ensheath synapses. B, Glutamate transporter currents recorded in an outside-out patch that was removed from a hippocampal astrocyte. The turnover rates of these native gluta mate transporters was estimated by measuring the time necessary for the peak of the response to 10mM L-glutamate to recover from the steady-state level. These results indicate that astrocyte transporters have a cycle time of approximately 10ms at physiological temperatures.