Overview of Embryonic Stem Cell Engineering Core Facility
Our Mission
| The ES Core Facility (ECF) was founded by the NINDS Core Center Grant and was established to benefit the contributors of this proposal. The mission of ECF is to effectively produce ES cell lines with a high probability of germline transmission. |
Core Service
Services provided by the Core for a typical project include:
- Provide guidance on the design of targeting construct
- Generate targeted ES cell lines for the production of chimeric mice
- Karyotyping ES cells to be micro-injected into blastocysts
Consultation is available from ECF directors and staff members on the entire procedures of generating gene knock-out mice.
Application for Service
Prior to the initiation of a project, a brief meeting is generally required between the investigator and ECF facility staff resulting in a mutually acceptable research strategy. This strategy will outline specifics of the project including knockout strategy, KO construct design, screening assays, and other procedural issues relevant to the generation of targeted ES cells. In addition, a completed service application form, signed by the principal investigator and approved by the Core Director, will also be required. The Core Director will prioritize the service requests according to the difficulty of the project and work load. |
Contact Information
Paul Worley M.D., Core Director, pworley@jhmi.edu; 410-955-4050
Alex Kolodkin, Ph.D., Center Director, kolodkin@jhmi.edu; 410-614-9499
Chip Hawkins, Core Manager, chawkins@jhmi.edu; 410-614-3858
For more information on The ES Core Facility