Overview of Monoclonal Antibody Core Facility
Staining of cultured hippocampal neurons with AMPA receptor antibodies |
Our Mission
The Monoclonal Antibody Core Facility (MACF) was founded by the NINDS Core Center Grant and was established to benefit the contributors of this proposal. The mission of (MACF) is to effectively produce high quality antibodies using high-throughput techniques.
[Publications presenting work supported by the Monoclonal Antibody Core] Location The Monoclonal Antibody Core is located in Room 913, Hunterian Building, at 725 North Wolfe St. The phone number is 410-955-4052. Support Min Dai, Core Manager, has many years experience with a wide variety of cell and molecular biology techniques, including extensive experience with techniques relevant to monoclonal antibody production such as mouse hybridoma cell fusion, cloning and screening of mouse monoclonal clones, and production of large quantities of monoclonal antibodies. Throughout his career, Dr. Rick Huganir (Core Director) has produced hundreds of antibodies against neuronal and synaptic proteins and many of them have been used by hundreds of investigators in the field of neuroscience. |
Core Service
Services provided by the Core for a typical project include:
Services provided by the Core for a typical project include: |
Consultation is available from MACF directors and staff members on peptide design, conjugation strategies and screening assays to ensure efficient detection of the desired monoclonal antibodies. Other antibody-related services may be available upon request (as time permits). |
Prior to the initiation of a project, a brief meeting is generally required between the investigator and MACF facility staff resulting in a mutually acceptable research strategy. This strategy will outline specifics of the project including antigen preparation, immunization protocols, supernatant screening strategies, and individual responsibilities. In addition, a completed service application form, signed by the principal investigator and approved by the Core Director, will also be required. The Core Director will prioritize the service requests according to the difficulty of the project and work load. |
Contact Information
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Richard L Huganir, Ph.D. Core Director rhuganir@jhmi.edu; 410-955-4050 |
Alex Kolodkin, Ph.D. Center Director kolodkin@jhmi.edu; 410-614-9499 |
Core Manager mdai1@jhmi.edu; 410-955-4052 |
