Overview of Monoclonal Antibody Core Facility
Staining of cultured hippocampal neurons with AMPA receptor antibodies |
Our Mission
The Monoclonal Antibody Core Facility (MACF) was founded by the NINDS Core Center Grant. The mission of the MACF is to effectively produce novel monoclonal antibodies that are directed against antigens of interest to NINDS investigators, providing a much- needed resource to the center’s primary investigators as well as other NINDS- funded scientists at JHU SOM. |
Core Service
Services provided by the Core for a typical project include:
Immunization of animals Testing mouse sera for positive response. Performing cell fusion ELISA screening Hybridoma cloning and subcloning Expansion and cryopreservation of antibody -positive hybridomas |
Consultation is available from MACF directors and staff members on peptide design, conjugation strategies and screening assays to ensure efficient detection of the desired monoclonal antibodies. Other antibody-related services may be available upon request (as time permits). |
Prior to the initiation of a project, a brief meeting is generally required between the investigator and MACF facility staff resulting in a mutually acceptable research strategy. This strategy will outline specifics of the project including antigen preparation, immunization protocols, supernatant screening strategies, and individual responsibilities. In addition, a completed service application form, signed by the principal investigator and approved by the Core Director, will be required. The Core Director will prioritize the service requests according to the difficulty of the project and workload. |
Contact Information
Richard L Huganir, Ph.D., Core Director
rhuganir@jhmi.edu; 410-955-4050
Alex Kolodkin, Ph.D., Center Director
kolodkin@jhmi.edu; 410-614-9499
Min Dai, Core Manager
mdai1@jhmi.edu; 410-955-4052