Bin Wu Ph.D.

Assistant Professor of Biophysics and Biophysical Chemistry

bwu20@jhmi.edu
Telephone Number: 410-502-4201
Fax Number: 410-955-0637

Johns Hopkins University School of Medicine
855 N. Wolfe Street
Baltimore, MD 21205
Room: Rangos 454
Areas of Research
Cellular + Molecular Neuroscience

Graduate Program Affiliations

Program in Molecular Biophysics

Biochemistry, Cellular and Molecular Biology Graduate Program

Neuroscience Training Program

Gene expression in neuron: seeing is belief


In neuron, gene expression has to be tightly controlled both temporally and spatially for its development and function. Localization of RNA to subcellular compartments such as dendrite and axon is a prominent way that neuron achieve asymmetric protein distribution and prompt response to stimulation. Dysfunction in local regulation of gene expression is one of the most common sources of neurodevelopmental and neurodegenerative disease. The Wu lab is interested in in visualizing and quantifying these gene expression events by monitoring the birth, transport, translation and decay of single mRNA molecules in live neurons. Recently we have developed a method called single molecule imaging of nascent peptides (SINAPS) to visualize translation of single mRNAs. With SINAPS, we can answer biological questions that are intractable before, such as the location of translation, the effect of synaptic function on local translation etc. The translation dynamics such as the initiation rate, translation speed can be monitored in live neurons. We are also interested in developing method to manipulate gene expression in live neurons. We hope to turn on or off gene expression anywhere in a cell.

 

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