Seth Blackshaw PhD

Professor of Neuroscience




Venkataraman A, Yang K, Irizarry J, Mackiewicz M, Mita P, Kuang K, Xue L, Ghosh D, Liu S, Ramos P, Hu S, Bayron D, Keegan S, Saul R, Colantonio S, Zhang H, Behn FP, Song G, Albino E, Asencio L, Ramos L, Lugo L, Morell G, Rivera J, Ruiz K, Almodovar R, Nazario L, Murphy K, Vargas I, Rivera-Pacheco ZA, Rosa C, Vargas M, McDade J, Clark BS, Yoo S, Seva G. Khambadkone5, de Melo J, Stevanovic M, Jiang L, Li Y, Yap WY, Jones B, Tandon A, Campbell E, Anderson S, Myers RM, Boeke JD, Fenyo D,  Whiteley G, Bader JS, Pino I, Eichinger DJ, Zhu H, and Blackshaw S.  A toolbox of immunoprecipitation-grade monoclonal antibodies against human transcription factors.  Nature Methods, in press.

Liu K, Kim J, Kim DW, Zhang S, Denaxa M, Bao H, Lim SA, Kim E, Liu C, Wickersham IR, Pachnis V, Hattar S, Song J, Brown SR, and Blackshaw S. Lhx6-positive GABAergic neurons of the zona incerta promote sleep.  Nature, 2017 548:582-587.

Liu S, Zibetti C, Wan J, Wang G, Blackshaw S, and Qian J.  BPAC: A universal model for prediction of transcription factor binding sites based on chromatin accessibility.  BMC Bioinformatics, 2017 18:355.

Cox E, Hwang W, Hu J, Guzzo C, Uzoma I, Matunis M, Qian J, Zhu H and Blackshaw S.  Global analysis of SUMO-binding proteins identifies SUMOylation as a key regulator of the INO80 chromatin remodeling complex.  Mol Cell Proteomics, 2017 16:812-823.

Bedont JL, LeGates TA, Buhr E, Bathini A, Ling J, Wong P, van Gelder R, Mongrain V, Hattar  S, and Blackshaw S. An Lhx1-Regulated Transcriptional Network Controls Sleep-Wake Coupling and Thermal Resistance of the Central Circadian Clockworks.  Current Biology, 2017 27:128-136.

De Melo J, Zibetti C, Clark BS, Hwang W, Miranda-Angulo AL, Qian J, Blackshaw S. Lhx2 Is an Essential Factor for Retinal Gliogenesis and Notch Signaling.   J Neurosci. 2016  36:2391-405.

Lagerlöf O, SlocombJE, HongI, Aponte Y, Blackshaw S, Hart GW and Huganir RL. The nutrient sensor OGT in PVN neurons regulates feeding.   Science, 2016 351:1293-6.

De Melo J,  Clark BS, and Blackshaw S. Multiple intrinsic factors act in concert with Lhx2 to direct retinal gliogenesis.  Scientific Reports, 2016 6:32757.

TheinT, de MeloJ, ZibettiC, ClarkBS, JuarezF, and Blackshaw S. Control of lens development by Lhx2-regulated neuroretinal FGFs.   Development, 2016, 143: 3994-4002.

Wang Y, An R, Umanah GK, Nambiar K, Park H, Eaker SM, Kim BW, Harraz MM,  Chang C, Chen R, Kam TI, Jeong JS, Xie Z, Neifert S, Qian J, Blackshaw S, Zhu H, Song H, Ming GL, Dawson VL, and Dawson TM. Identification of the PARP-1 dependent AIF-associated nuclease.  Science, 2016 354: pii: aad6872.

Blackshaw S, Anderson S, Campbell E, Freeman N, Gatlin C, Pino I, Zhu H and Bader J.The NIH Protein Capture Reagents Program (PCRP): a standardized affinity reagent toolbox.  Nature Methods, 2016 13:805-6.

Mattar P, Ericson J, Blackshaw S, and Cayouette M. A conserved regulatory logic controls temporal identity in mouse neural progenitors.  Neuron, 2015  85:497-504.

Lee DA, Yoo S, Pak T, Salvatierra J, Velarde E, Aja S, and Blackshaw S.  Dietary and sex-specific factors regulate hypothalamic neurogenesis in young adult mice.  Frontiers in Neuroscience, 2014  8:15.

Salvatierra J, Lee DA, Zibetti C, Duran-Moreno M, Yoo S, Newman EA, Wang H, Bedont JL, de Melo J, Miranda-Angulo AL, Gil-Perotin S, Garcia-Verdugo JM, and Blackshaw S. The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation.  J Neurosci, 2014 34:16809-20.

Bedont JL, LeGates TA, Slat EA , Byerly MS, Wang H, Hu J, Rupp AC, Qian J, Wong GW, Herzog ED, Hattar S, and Blackshaw S.  Lhx1 controls terminal differentiation and circadian function of the suprachiasmatic nucleus. Cell Reports, 2014 7:609-22.

Lee DA, Yoo S, Pak T, Salvatierra J, Velarde E, Aja S, and Blackshaw S.  Dietary and sex-specific factors regulate hypothalamic neurogenesis in young adult mice.  Frontiers in Neuroscience, 2014 8:15.

Pak T, Yoo SY, Miranda-Angolo AM, Wang H, and Blackshaw S.  Rax-CreERT2 knock-in mice: a tool for selective and conditional gene deletion in progenitors and radial glia of the retina and hypothalamus. PLoS ONE, 2014, 9:e90381.

Liu S,  Lamaze A,  Liu Q, Tabuchi M, Yang Y, Fowler M, Bharadwaj R, Zhang J, Bedont JL, Blackshaw S, Lloyd TE, Montell C, Sehgal A, Koh K, and Wu MN.  WIDE AWAKE mediates the circadian timing of sleep onset.  Neuron, 2014 82:151-6.

Byerly MS, Swanson R, Kwon K, Aja S, Moran TH, Wong GW and Blackshaw S.  Hypothalamic Neuron-derived Neurotrophic Factor (NENF) interacts with BDNF and melanocortin signaling to modulate food intake.  American Journal of Physiology, (2013) e-pub ahead of print.

Roy A, de Melo J, Chaturvedi D, Thein T, Cabrera-Socorro A, Meyer G, Blackshaw S, and Tole S.  Lhx2 is necessary for the maintenance of optic identity and for the progression of optic morphogenesis.  J Neurosci, (2013) 33:6877-8

Lee DA, Bedont JL, Pak T, Wang H, Song J, Miranda-Angulo A, Takiar V, Charubhumi V, Balordi F, Takebayashi H, Ford E, Fishell G, and Blackshaw S. Tanycytes of the Hypothalamic Median Eminence Form a Diet-Responsive Neurogenic Niche.  Nat Neurosci (2012), 15:700-2 (Highlighted in Nature Neuroscience, May 2012 and Nature Cell Biology, October 2012).

De Melo J, Miki K, Rattner A, Smallwood P, Zibetti C, Hirokawa K, Monuki ES, Campochario P, and Blackshaw S. Injury-independent induction of reactive gliosis in retinal Muller glia by loss of function of the LIM homeodomain transcription factor Lhx2.    Proc Natl Acad Sci USA (2012) 109:4657-62.

Jeong JS, Jiang L, Albino E, Marrero J, Rho HS, Hu S, Woodard C, Vera C,  Bayron-Poueymirou D, Rivera-Pacheco ZA, Ramos L, Torres-Castro C, Bonaventura J, Boeke JD, Pino I, Eichinger DJ, Zhu H and Blackshaw S.  A human proteome microarray-based pipeline for efficient production of monospecific monoclonal antibodies.  Molecular Cellular Proteomics (2012) 6;O111.016253.

Rapicavoli NA, Poth EM, Zhu H and Blackshaw S.  The long noncoding RNA Six3OS acts in trans to regulate retinal development by modulating Six3 activity.  Neural Development (2011) 6:23

De Melo J, Peng G-H, Chen S and Blackshaw S.   Sall3 controls retinal cone photoreceptor and horizontal cell development.  Development (2011) 138:2325-36. 

Blackshaw S, Scholpp S, Placzek M, Ingraham H, Simerly R, and Shimogori T.  Molecular pathways controlling development of thalamus and hypothalamus: from neural specification to circuit formation.  J Neurosci.(2010) 30:14925-30.

Shimogori T, Lee D A, Miranda-Angulo A, Yang Y, Yoshida A, Jiang L, Kataoka A, Wang H, Mashiko H,  Avetisyan M A, Qi L, Qian J, and Blackshaw S.  A genomic atlas of mouse hypothalamic development. NatNeurosci (2010) 13:767-75. (Highlighted in Nature Neuroscience, June 2010 and Faculty of 1000).

Rapicavoli N, Poth E, and Blackshaw S.  The long noncoding RNA RNCR2 directs mouse retinal cell specification.  BMC Developmental Biology (2010) 10:49. PMC2876091

Onishi A, Peng GH, Chen S, Blackshaw S. Pias3-dependent SUMOylation controls mammalian cone photoreceptor differentiation. Nat Neurosci (2010) 13:1059-65. 

Onishi A, Peng G-H, Chen J, Lee DA, Alexis U, Poth E, de Melo J, Chen S, and Blackshaw S.  The orphan nuclear hormone ERRb regulates rod photoreceptor development and survival.  Proc Natl Acad Sci USA (2010) 107:11579-84.

Hu S, Xie Z, Onishi A, Jiang L, Wang H, He X, Rho H-S, Woodard C, Yu X, Lin J, Long S, Blackshaw S*, Qian J*, and  Zhu H*.  Profiling the human protein-DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling.  Cell (2009) 139:610-22. (Highlighted in Cell, October 2009 and Faculty of 1000.  *indicates corresponding author).

Onishi A, Peng GH, Du CH, Alexis U, Chen S, and Blackshaw S. Pias3 directs rod photoreceptor development via SUMOylation of Nr2e3.  Neuron  (2009) 61:234-46. (Highlighted in Neuron, January 2009 and Faculty of 1000).

Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho S-H, Yung R, Asch E, Wong, WH, and Cepko CL   Genomic analysis of mouse retinal development.  PLoS Biol. (2004) 2:E247.

Blackshaw S, Fraioli RE, Furukawa, T, and Cepko, CL  Comprehensive analysis of photoreceptor gene expression and the identification of candidate retinal disease genes.  Cell, (2001) 107:579-89. (Highlighted in Cell, November 2001 and Faculty of 1000).